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Conformational changes of the glucocorticoid receptor ligand binding domain induced by ligand and cofactor binding, and the location of cofactor binding sites determined by hydrogen/deuterium exchange mass spectrometry

机译：配体和辅因子结合引起的糖皮质激素受体配体结合域的构象变化，以及氢/氘交换质谱法测定的辅因子结合位点的位置

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HXMS (hydrogen/deuterium exchange mass spectrometry) of the glucocorticoid receptor ligand-binding domain (GR LBD) complexed with the agonist dexamethasone and the antagonist RU-486 is described. Variations in the rates of exchange were observed in regions consistent with the published crystal structures of GR LBD complexed with RU-486 when compared with the GR dexamethasone complex. We also report the HXMS results for agonist-bound GR LBD with the coactivator transcriptional intermediary factor 2 (TIF2) and anatagonist-bound GR LBD with nuclear receptor corepressor (NCoR). Alterations in exchange rates observed for agonist-bound GR LBD with TIF2 present were consistent with the published crystal structural contacts for the complex. Alterations in exchange rates observed for antagonist-bound GR LBD with NCoR were a subset of those observed with TIF2 binding, suggesting a common or overlapping binding site for coactivator and corepressor.

机译：描述了与激动剂地塞米松和拮抗剂RU-486复合的糖皮质激素受体配体结合域（GR LBD）的HXMS（氢/氘交换质谱）。当与GR地塞米松复合物比较时，在与RU-486复合的GR LBD的已公布晶体结构一致的区域中观察到交换速率的变化。我们还报告了激动剂结合的GR LBD与辅激活因子转录中介因子2（TIF2）和拮抗剂结合的GR LBD与核受体corepressor（NCoR）的HXMS结果。对于存在TIF2的激动剂结合的GR LBD，观察到的汇率变化与该复合物的已公布晶体结构接触相一致。用NCoR观察到的与拮抗剂结合的GR LBD的交换速率变化是观察到的与TIF2结合的交换率的一个子集，表明共激活因子和共加压因子具有共同或重叠的结合位点。

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Frego, Lee; Davidson, Walter;
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年度 2006
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1. Conformational changes of the glucocorticoid receptor ligand binding domain induced by ligand and cofactor binding, and the location of cofactor binding sites determined by hydrogen/deuterium exchange mass spectrometry. [J] . Frego L, Davidson W Protein Science: A Publication of the Protein Society . 2006,第4期

机译：配体和辅因子结合引起的糖皮质激素受体配体结合域的构象变化，以及氢/氘交换质谱法测定的辅因子结合位点的位置。
2. Ligand Binding Domain Mutations of the Glucocorticoid Receptor Selectively Modify the Effects with, but Not Binding of, Cofactors [J] . Geun-Shik Lee and S. Stoney Simons Jr. Biochemistry . 2011,第3期

机译：糖皮质激素受体的配体结合域突变选择性修饰了辅因子的作用，但未结合辅因子
3. Ligand Binding Domain Mutations of the Glucocorticoid Receptor Selectively Modify the Effects with, but Not Binding of, Cofactors [J] . Lee GS, Simons SS Biochemistry . 2011,第3期

机译：糖皮质激素受体的配体结合结构域突变选择性地改变官方acactors的助剂，但不具有辅助act
4. Determination of Site-specific Protein-Ligand Binding Affinities using Protein Ligand Interaction by Mass Spectrometry, Titration, and Hydrogen/Deuterium EXchange (PLMSTEX) Strategy [C] . Jagat Adhikari, Don L. Rempel, Michael L. Gross ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：用质谱法测定蛋白质配体，滴定和氢气/氘交换（PLMSTEX）策略的蛋白质配体相互作用测定特异性蛋白质 - 配体结合亲和力
5. Probing protein small ligand binding dynamics by hydrogen/deuterium exchange and electrospray ionization mass spectrometry. [D] . Xiao, Hui. 2005

机译：通过氢/氘交换和电喷雾电离质谱探测蛋白质小配体结合动力学。
6. Conformational changes of the glucocorticoid receptor ligand binding domain induced by ligand and cofactor binding and the location of cofactor binding sites determined by hydrogen/deuterium exchange mass spectrometry [O] . Lee Frego, Walter Davidson 2006

机译：配体和辅因子结合引起的糖皮质激素受体配体结合域的构象变化以及氢/氘交换质谱法测定的辅因子结合位点的位置
7. Ligand Binding Domain Mutations of the Glucocorticoid Receptor Selectively Modify the Effects with, but Not Binding of, Cofactors [O] . Geun-Shik Lee, S. Stoney Simons 2011

机译：糖皮质激素受体的配体结合结构域突变选择性地改变辅助剂的辅助剂的效果

Conformational changes of the glucocorticoid receptor ligand binding domain induced by ligand and cofactor binding, and the location of cofactor binding sites determined by hydrogen/deuterium exchange mass spectrometry

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